Research on the interaction of protein Tmub1 and securin after partial hepatectomy

Xiao-biao ZHAO, Guang-yao LI, Meng-gang LIU, Xia FAN, Ping CHEN

Abstract


Objective To investigate the interaction of Tmub1 and securin protein after partial hepatectomy. Methods  A total of 30 adult male SD rats were randomly divided into 3 groups (10 each) according to the time after hepatectomy, i.e, 0, 24 and 48 h. Partial hepatectomy was performed in the rats in the first group, and then the primary hepatocytes were directly harvested by liver perfusion. Partial hepatectomy was also performed in the rest two groups at postoperative 24 and 48 h. Then the primary hepatocytes were also harvested respectively after liver perfusion. One part of primary hepatocytes was cultured for one night and for the observation of the colocalization of Tmub1 and securin protein with immunofluorescence. The other part of hepatocytes were used for co-immunoprecipitation and Western blotting to detect the expression of protein Tmub1/securin. Results Immunofluorescence and confocal laser scanning microscopy showed that there were almost no Tmub1 and securin proteins in the nucleus 0 h after liver resection, while a great amount of Tmub1/securin proteins were found to be co-localized in the nucleus 24 and 48 h. Coimmunoprecipitation and Western blotting showed that the expression of Tmub1/securing protein significantly enhanced in 24-48 h after operation compared with that at 0 h (P0.05). Conclusion Tmub1 hardly combines with securin protein in normal hepatocytes, but it combines with securin protein when it migrates from cytoplasm into nucleus after hepatectomy, thus inhibiting the proliferation process of hepatocytes.

DOI: 10.11855/j.issn.0577-7402.2014.11.04

Keywords


Tmub1 protein; securin protein; liver regeneration; hepatectomy

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